Development of Single Particle Irradiation to CEll system in NIRS

Abstract

Bystander Effect is one of the interesting researches in the field of low dose radiation effect. It occurs due to some unknown response between the directly irradiated cell and normal ones. For the study of Bystander Effect, a microbeam cell irradiation system becomes a strong tool. SPICE (Single Particle Irradiation system for Cell) of NIRS was constructed and we start its operation in 2003. On the SPICE, some newly developed systems are mounted. One is a microscope system which is consists of OLYMPUS fluorescent microscope parts mounted on a granitic level block, and high performance voice coil motor system. And the other is single ion hit system consisted from single ion counter, signal control PC board, high-voltage pulse generator, and electrostatic beam deflector. In this conference, we'll make a presentation about these developments, and test examination of cell. Additionally, we'll show the development of new type cell dish use for accurate targeting of beam.