Journal of Surface Analysis
Online ISSN : 1347-8400
Print ISSN : 1341-1756
ISSN-L : 1341-1756
Technical Report
A simple preparation method for observation of aquatic micro-organisms by scanning electron microscope
M. InoueM. Suganami
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2012 Volume 19 Issue 2 Pages 81-84

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Abstract
For observation of aquatic micro-organisms by a scanning electron microscope, sample pretreatments are necessary to avoid the deformation of cell membrane by evaporation of moisture from inside of the cell in vacuum and also the charging-up of sample under irradiation of electron beam. In the present paper, we report on a simple preparation method by ethanol dehydration and the ionic liquid coating method without using any apparatus for preparation such as a refrigerator, a vacuum equipment and etc.
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© 2012 by The Surface Analysis Society of Japan
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