Adsorption behaviors of γ-lobulin on poly (2-hydroxyethyl methacrylate) (PHEMA), a hydrophilic surface, poly-styrene (PSt), a hydrophobic surface and HEMA-St block copolymer, microphase separated surface were studied by UV-CD method, wettability of protein solution and newly method of electron microscopy using ferritin labelled rabbit anti-human γ-globulin IgG as a staining agent. These results showed the different orientation of adsorbed γ-globulin on the hydrophilic and the hydrophobic surface. That is, γ-globulins were adsorbed onto the hydrophobic surface with therir Fc segment, but adsorbed onto the hydrophilic surface with their Fab segment. These data suggest that the deformation of the adhered platelets and lymphocytes was large on the γ-globulin precoated PHEMA surface because of specific reaction of platelet and lymphocyte Fc receptor with Fc segment which is exposed to liquid phase. On the contraly, γ-globulin selectively adsorbed on the hydrophobic domains of block copolymer surface. So it was elucidated that the adhered platelets and lymphocytes were observed to be round.
