The high speed liquid chromatography (HSLC) has been commonly used for the analysis of middle molecule substances (MM). This is because of easily detecting many materials in a serum which are sensitive to various detectors. The deproteined serum of the patient on routine maintemance hemodialysis was analyzed with HSLC and the parameters in the two pool model were then determined. Two unknown paramerters such as cell membrane clearance (Kc) and solute generation rate (G) could be estimated by the trial & error method on a computer. The input of these postulated values into the equations for the pool model is adjusted until a best fit to the data from HSLC analysis is found.
The value of Kc for MM peaks was found to be very small (Table 2). In the case of No, 1, MM concentration in peak a5 is very sensitive to clearance (CL).
MM is more suitable for a marker showing the extent of rejection on kidney transplantation than creatinine so far used clinically.
