ADSORPTION AND REMOVAL OF ENDOTOXIN BY POLYMYXIN-SEPHAROSE, ITS PROPERTIES AND APPLICATIONS

M. UMEDA; M. ASAKAWA; T. TERADA; Y. TATSUMI; N. KASHIWARA; J. KAWAKITA; S. YAMAGAMI; T. KISHIMOTO; M. MAEKAWA; M. NIWA

doi:10.11392/jsao1972.12.275

M. UMEDA, M. ASAKAWA, T. TERADA, Y. TATSUMI, N. KASHIWARA, J. KAWAKITA, S. YAMAGAMI, T. KISHIMOTO, M. MAEKAWA, M. NIWA

著者情報

M. UMEDA
Osaka City Univ. Med. Sch., Dept, of Artificial Organs
M. ASAKAWA
Osaka City Univ. Med. Sch., Dept, of Artificial Organs
T. TERADA
Osaka City Univ. Med. Sch., Dept, of Artificial Organs
Y. TATSUMI
Osaka City Univ. Med. Sch., Dept, of Artificial Organs
N. KASHIWARA
Osaka City Univ. Med. Sch., Dept, of Artificial Organs
J. KAWAKITA
Osaka City Univ. Med. Sch., Dept, of Artificial Organs
S. YAMAGAMI
Osaka City Univ. Med. Sch., Dept, of Artificial Organs
T. KISHIMOTO
Osaka City Univ. Med. Sch., Dept, of Artificial Organs
M. MAEKAWA
Osaka City Univ. Med. Sch., Dept, of Artificial Organs
M. NIWA
Dept. of Bacteriol.

ジャーナルフリー

1983 年 12 巻 1 号 p. 275-278

DOI https://doi.org/10.11392/jsao1972.12.275

詳細

抄録

How polymyxin-B binds to and inactivates endotoxin was studied. Antibacterial property of 30μg of PX was inactivated by 500μg of ET (extracted from S. min R. 595), while the biological activity of 4μg of ET was inactivated completely by 40μg of PX. Then, PX was immobilized on CNBr activated Sepharose by covalent bond. (PX-Seph). 680μg of stabilized PX could remove 40μg of ET by adsorption. Such effect was found also in plasma. Affinity constant (Ka) in this binding showed a high value of 4.06 x 10⁸.

責任著者(Corresponding author)

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