Computer Analysis of HPLC data in Serum

Abstract

High performance liquid chromatography (HPLC) combined with data processor (Personal computer: PC-8801) was utilized to characterize blood purification therapy, disease condition and organ function. The two types of HPLC colums supplied by A sahi Medical Co., Ltd. were tested to show us various peaks of high molecular weight compounds and low or middle molecular weight compounds in sera or plasma. Among them, albumin globulin peaks separations were so far found clinically effective; human nonmercaptalubumin (HNA) was observed in most cases of underdialysis and critical illness while the HNA was decreasing with uremic toxins decrease due to various blood purification therapies. Our method of HPLC was concluded useful to characterize disease condition and blood purification therapy.