Abstract
PMMA and PAN membranes have adsorptive characteristics for proteins. We reported that PMMA membrane had strong adsorptive capacity for β2-microglobulin (β2-MG), retinol-binding protein and IgG compared with PAN membrane, and also these membranes had different characteristics for protein adsorption (Jpn J Artif Organs 19, 661, 1990). To clarify the further characterizations of PMMA and PAN membranes for protein adsorption, used membranes for hemodialysis were perfused with 8M urea solution and washed with the perfusate adding 10% DMSO in ultrasonic cleaner. The perfusates were analyzed with two-dimensional electrophoresis(2-DE), cellulose acetate electrophoresis(CAE) and gel filtration. 2-DE of PMMA membrane perfusate showed β2-MG spot remarkably on polyacrylamide gel, which was similar to our previous report. CAE and gel filtra-tion studies showed different pattern between PMMA and PAN membrane perfusates. We concluded that PAN membranes had different characteri stics in protein adsorption fromPMMA membranes.
