Abstract
Severe hemolysis may cause the various complications, such as acute renal failure. In these cases, if free hemoglobin could be removed promptly from the body, the risk might be lowered.
For this purpose, we have devised a filter containing immobilized haptoglobin which was used as affinity adsorbent of free hemoglobin. Haptoglobin, which was prepared from human plasma in our group, was immobilized to human fibrin polymer formed by fibrinogen-fibrin conversion in the presence of thrombin.
The results were as follows:
1. Fibrin particles (50-150u) kept the more suitable solidity than sepharose particles in circulation.
2. Haptoglobin binding capacity of fibirn was 460mgHp/g under 5mMol glutaraldehyde, but 180 mgHp/g under no glutaraldehyde. This capacity decreased under the high concentration of glutaraldehyde.
3. Hemoglobin adsorbent capacity of fibrin immobilizes haptoglobin was 58 mgHb/g in vitro, and about 30mgHb/g (1.7mgHb/ml) in extracorporeal circulation on rabbit.
This study suggested the possible use of immobilized haptoglobin. Now, we are trying to develop more practical application.
