Collagen-coated synthetic polymer, a biomaterial which possesses high tissue compatibility, has been shown to be adequately applicable for a support of the immobilization of trypsin and urokinase. The enzymes were successfully bound to the collagen membrane activated by acyl azide formation from its carboxyl groups. The urokinase-bearing collagen-coated synthetic polymer showed fibrinolytic activity on fibrin-agarose plate. Thus, the enzyme immobilized onto the surface of collagen provides the material with some biological functions. In view of high plasticity of synthetic polymers, an enzyme-collagen-polymer complex will find wide applications for biomaterials and artificial organs.
