This report was investigated to establish the more effective immobilization method which was able to produce the stronger fibrinolytic activity in nylon immobilized urokinase.
For this purpose, we designed five procedures that urokinase was immobilized to nylon (1) without any treatment, (2) after the etching of 3N HCI (3) through the grafted copolymer such as polyethy leneimine and (3) gantrez, and (5) under the combination of linking of nitrophthalic acid. The relative merits of these each procedures were examined on a view-point of the intensity of fibrinolytic activity the stability of repeated fibrinolysis and the influence of disinfection.
From these experiments, the available immobiliza tion methods were the procedures of N/PEI/G/UK and N/PEI/G: NP/UK.
