This report was investigated to establish the more effective disinfection method for nylon immobilized urokinase.
For this purpose, we chose the three types of disinfection ((1) 0.5% chlorhexidine alcohol·5min, (2) boiling 100°C·20min, (3) γ-rays 100×104 rad) and designed the three types of nylon immobilized urokinase ( (a) disinfected lastly…… N/PEI/G: NP/UK, (b) again immobilized urokinase after the disinfection of completed material which had been immobilized urokinase…… N/PEI/G: NP/UK+UK, (c) first immobilized urokinase after the disinfection of incompleted material which was not immobilized urokinase yet……N/PEI/G: NP+UK).
From these experiments, the available disinfection methods were (1) and (3). We can especially commend the combination method of (3) and (c). On binding form, we suppose that the covalent binding such as peptide linking and the stable ionic binding are main form.
