抄録
The artificial biomedical materials on which antithrombin III and heparin were co-immobilized (I-AT III·HEP) had the significant anticoagulative activity. We observed the prolongation of plasma recalcification time and the activities of anti-thrombin and anti-activated factor X by I-AT III HEP in vitro. I-AT III·HEP showed the higher rate of neutralization of activated factor X than that of thrombin. By the reaction of the protease enzymes with I-AT III·HEP, the more quantity of activated factor X was neutralized than thrombin neutralized. The biomedical materials on which antithrombin III was immobilized in the presence of excess of acetylated heparin (I-AT III) could neutralize activated factor X and thrombin. However, the degree of anti-coagulative activity by I-AT III was slight, compared with I-AT III·HEP. Heparin immobilized on biomedical materials (I-HEP) was able to be combined to thrombin tightly and combine to activated factor X by affinity, but enzymes were not able to be neutralized directly. Co-immobilization of antithrombin III and heparin was applied to silicone which contained hydroxyl groups and it showed good anti-coagulative activity.
