Algal Resources
Online ISSN : 2423-8473
Print ISSN : 1883-3284
A simple pretreatment and HPLC method for β-carotene determination in edible seaweeds
Hirotaka KAKITA Hideki OBIKA
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2017 年 10 巻 2 号 p. 59-66

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To develop a simple pretreatment and reversed-phase chromatography method for β-carotene in edible seaweeds, optimum HPLC column and high efficient extraction method were selected. Beta-carotene measurement was performed at 450 nm. A TSKgel ODS-80TS column seems to be a suitable column for β-carotene analysis among three columns tested. Suitable extraction solvent for high efficiency extraction was ethanol containing 10% pyrogallol among extraction solvents tested. The present method was successfully applied to β-carotene analysis and should prove useful for β-carotene analysis in edible seaweeds.
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© 2017 The Japanese Society of Applied Phycology
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