2014 年 7 巻 2 号 p. 95-105
To identify the cultivable epiphytic bacteria obtained from the unialgal culture strain of the red alga Gracilariopsis chorda (Holmes) Ohmi collected from the estuary of Katsuura River in Tokushima Prefecture in southwest Japan, morphological and phenotypic classification and genetic analysis were carried out. Eight kinds of cultivable bacteria were isolated. Isolate UGC1-1 (bacterial population: 7.5×107 cfu g-1), UGC1-2 (4.0× 107 cfu g-1) and UGC1-3 (5.0× 106 cfu g-1) were isolated from Marine Agar plates. Isolate UGC1-4 (5.0×106 cfu g-1) and UGC1-5 (1.0×106 cfu g-1) were isolated from 1/10 ORI and CAM plates, respectively. Isolate UGC1-6 (1.0×105 cfu g-1), UGC1-7 (6.0×104 cfu g-1) and UGC1-8 (2.0×104 cfu g-1) were isolated from CVT plates. The genetic analysis based on partial 16 SrRNA gene sequences (ca. 450 bp) revealed that the Isolate UGC1-1, UGC1-2, UGC1-3, UGC1-4, UGC1-5, UGC1-6, UGC1-7 and UGC1-8 related closely to Ascidianbacter aurantiacus (Similarity 93.2%), Ruegeria halocynthiae (99.5%), Roseovarius sediminilitoris (97.2%), Litoreibacter janthinus (99.3%), Zobellia russellii (99.8%), Marinobacter salarius (99.1%), Granulosicoccus antarcticvs or G. coccoides (97.9%) and Labrenzia aggregata (99.8%), respectively. The Isolate UGC1-1 was suggested to be an undescribed taxon assigned to the family Flavobacteriaceae based on genetic analysis.
