Normal phase partition chromatography for monosaccharide and disaccharide analysis in three kinds of green laver powder

抄録

To develop a simple rapid technique in normal phase partition chromatography for monosaccharide and disaccharide analysis in edible seaweeds, a comparison between a TSKgel NH₂-100 column packed with chemically bonded amino-silica gel and a TSKgel Amide-80 column, also packed with chemically bonded amide-silica gel, was carried out. Saccharide chromatographic behavior, resolution, and elution rapidity were evaluated. The anomeric peaks of reducing saccharides split at 25 ℃ and 40 ℃ in a TSKgel Amide-80 column, although they did not split at 40 ℃ in a TSKgel NH₂-100 column. An increase of acetonitrile concentration in the mobile phase increased the saccharide retention times. Usually, but not always, increasing the hydroxyl group of saccharides tends to increase the retention time. Saccharide retention times increased mostly in order as follows: deoxyhexose＜pentose＜ketohexose＜aldohexose. Saccharide resolution and peak heights in a TSKgel NH₂-100 column were higher than those in a TSKgel Amide-80 column. Thus, a TSKgel NH₂-100 column was used as the column for saccharide analysis in this study. The present technique was successfully applied to monosaccharide and disaccharide analysis and should be useful for monosaccharide and disaccharide analysis in edible seaweeds.