A microfluidic method for production of monodisperse liposomes

抄録

Liposomes are lipid vesicles with a cell-like bilayer membrane structure that are used to construct artificial cell models. To ensure the reproducibility of experiments and enable quantitative analysis, a method for producing liposomes with uniform size and high encapsulation efficiency is required. Recently, we developed a system for generating monodisperse liposomes using a microfluidic device. This system can produce monodisperse liposomes with diameters ranging from 25 to 45 µm, achieving a yield of approximately 100%. To apply this system to the construction of artificial cells, we present the results of encapsulating an in vitro transcription/translation system in liposomes to investigate the conditions under which protein synthesis occurs.