Abstract
As reported in a previous paper, intraperitoneally infected virulentSalmonella enteritidisorganisms were promptly cleared from the abdominal cavity of recipient mice which had been transferred with mesenchymal cells of donor mice hyperimmunized with killed organisms of the same strain. The effectiveness of of transfer, however, disappeared within a week or so.
In the present study, we attempted to clarify the mechanism of disappearance of transfer effect with the following results:
1. In the suckling mice transferred withhomologous immune cells within 12 hrs. after birth, the transfer effect was found throughout the experimental period of 27 days.
2. When the transfer was made among the adult mice of an inbred strain dd/Ks its effectiveness continued for more than 110 days after transfer.
3. A temporary ineffectiveness of transfer was frequently observed, when the cells were transferred into 20 to 25 day old mice, This unexpected result was assumed to be due to decreased concentration of gamma globulin in recipients of those ages, which had compelled them to consume not only normal gamma globulin but also the adoptively formed antibody.
4. In the recipient mice pretreated with either cortisone or total body irradiationwith 400 r of Xray, the transfer effect was observable for more than 20 days.
5. The effectiveness of transfer disappeared within 3 days, when the recipients had been sensitizedwith homologous normal cells 12 days prior to transfer. All the above results seemed to support the assumption that the disappearance of the effectiveness of of transfer may be ascribable to the development in recipients of homotransplantation immunity against the transferred cells.
