Abstract
Lyophilized material, Dried Tuberculin or DT, prepared from the unheated and concentrated culture filtrate of human strain Aoyama-Bwas fractionated by the use of Zone electrophoresis employing starch as the suporting medium, and 4 fractions, i. e. one polysaccharide and 3 protein fractions I, II, and III were obtained. Each of them was electrophoretically single component.
The protein fractions II and III were almost consisted of proteins and contained very small amount of other components. There was no remarkable difference between the fractions II and III in their chemical properties. The protein fraction I contained polysaccharide in almost the same amount as protein.
