Nippon Saikingaku Zasshi

Relationship between the Intracellular Multiplication of Salmonellae in Cultured Mononuclear Phagocytes and their Pathogenicity for the Host

II. Experiments with the Rabbit

Daizo USHIBA, Masayasu NAKANO, Kazuhisa SAITO, Takehisa AKIYAMA, Fujio ...

1963 Volume 18 Issue 2 Pages 65-70
Published: February 25, 1963
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.18.65

JOURNAL FREE ACCESS

Show abstractHide abstract

The in vitro intracellular growth of various species of salmonellae tested by the roller tube technique. using rabbit peritoneal macrophages was compared with their pathogenicity which was examined by intravenous and intraperitoneal inoculations into rabbits. Strains of S. choleraesuis, S. enteritidis, S. typhimurium and S. paratyphi B showed apparent intracellular growth, while no growth could be observed with S. paratyphi A, S. typhosa and Escherichia coli (0-55). Among the former strains, S. paratyphi B showed a particular microscopic finding in which no marked degeneration or destruction of cells occured in spite of the rapid multiplication of organisms. When each one strain of the 4 species which multiplied intracellularly was inoculated into rabbits, only S. choleraesuis elicited typical typhoid infection with high fever and bacteremia, while the others showed only a slight pathogenicity with temporary fever and antibody formation. The granuloma formation in the liver was noted in rabbits inoculated with the 4 species. No sign of infection and pathological change could be found in rabbits inoculated with S. paratyphi A.

View full abstract

Download PDF (839K)
Fractionation of human strain Aoyama-B tuberculin by the use of Zone electrophoresis

1. Fractionation procedures and the chemical properties of the fractions

Tetsuro KATAOKA

1963 Volume 18 Issue 2 Pages 71-74
Published: February 25, 1963
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.18.71

JOURNAL FREE ACCESS

Show abstractHide abstract

Lyophilized material, Dried Tuberculin or DT, prepared from the unheated and concentrated culture filtrate of human strain Aoyama-Bwas fractionated by the use of Zone electrophoresis employing starch as the suporting medium, and 4 fractions, i. e. one polysaccharide and 3 protein fractions I, II, and III were obtained. Each of them was electrophoretically single component.
The protein fractions II and III were almost consisted of proteins and contained very small amount of other components. There was no remarkable difference between the fractions II and III in their chemical properties. The protein fraction I contained polysaccharide in almost the same amount as protein.

View full abstract

Download PDF (762K)
A simple and sterile isolation method of Myc. lepraemurium from infected tissues

Masahiro NAKAMURA, Yoshie UENO

1963 Volume 18 Issue 2 Pages 75-79
Published: February 25, 1963
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.18.75

JOURNAL FREE ACCESS

Show abstractHide abstract

A simple method for isolation and purification of Myc. lepraemurium from the infected tissues by treatment of trypsin (final concentration: 0.2%) with sodium desoxycholate (final concentration: 0.1%) was described. This procedure can be performed under sterile conditions and is harmless for the infectivity of the bacilli. The tissue component can be almost completely removed from the homogenate.

View full abstract

Download PDF (3874K)
[title in Japanese]

1963 Volume 18 Issue 2 Pages 81-107
Published: February 25, 1963
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.18.81

JOURNAL FREE ACCESS

Download PDF (5900K)

Register with J-STAGE for free!