抄録
Factors influencing synthesis of alpha toxin by Cl. perfringens PB6K, were investigated by using a synthetic medium supplemented with a peptide (glycyl-L-asparagine) or a fraction derived from a protein. digest. Among various factors studied, amounts and kinds of phosphate, reducing agent and sugar seemed to affect greatly the yield of toxin. As a reducing agent, thioglycolic acid was by far superior to ascorbic acid for toxin production, while the latter was more suitable for growth. Regarding phosphate, a mixture. of Na2HPO4 and KH2PO4 was more favourable for toxin production than that of K2HPO4 and KH2PO4, while the latter supported a better growth. Fructose was preferable to sucrose or glucose so far as the yield of toxin was concerned.
