抄録
Presented in this paper were the successful induction experiments of lysogenic mycobacteria, Myc. sp. Li (L1), by the use of ultraviolet light or hydrogen peroxide. Special sensitivity of this temperate phage to the different kinds of media was effectively utilized in the present experiments.
1. Phage Li released from Myc. sp. Li (L1) was rapidly inactivated in a M/15 phosphate buffer or physiological saline, but not in broth, distilled water and Tris buffer. Addition of more than 0.002 M CaCl2 prevented the inactivation. Inactivated phage Li was, however, not reactivated by the addition of Ca.
2. Myc. sp. L1 (L1) was successfully induced by the ultraviolet irradiation. Latent period after the induction was about 120-150 minutes, rise period about 150 minutes and burst size was calculated as about 5.
3. Myc. sp. L1 (L1) was successfully induced by hydrogen peroxide, too. Maximum induction rate was observed 8 minutes after the addition of hydrogen peroxide.
