Abstract
For the purpose of determining the pathogenicity of Vibrio parahaemolyticus, the author intended to made 2 experiments on the bacterial cultures derived from the same strains. viz., 1) The oral administration test of V. parahaemolyticus in monkeys. 2) The De-test, a unique inoculation technique using a live rabbit small intestine. In this test the animals were sacrificed at the certain intervals of time, then observed both visualy and histopathologically.
The results obtained are as follows:
1) None of 14 monkeys showed any clinical symptoms suggesting enteritis, which occured in the case of human enteritis caused by this organisms.
2) In the autopsy of the De-test on the rabbit, it is observed that the exudate began to accumurate in the ligated loop of the intestinal tract, at about 6 hours post-inoculation and remarkable edema was appeared. The degeneration of the mucous membrane cells were observed histopathologically at about 9-12 hours, followed by the necrotic changes.
3) The results of De-test observed at 24 hours post inoculation of V. parahaemolyticus were resembling to that of induced by El Tor vibrio.
4) It is presumable from the results of De-test that the pathogenic substance of this organism is a relatively thermolabile component of the bacterial cell.
5) It was clarified that the De-test is one of the useful procedures for judging the pathogenicity of V. parahaemolyticus.
