Abstract
From 8 strains of Candida, C. albicans A-I, C. albicans A-II, C. albicans B, C. tropicalis, C. stellatoidea, C. guilliermondii, C. parakrusei, and C. krusei, polysaccharides were extracted with hot aqueous alkali and purified by the precipitation with Fehling solution.
The purified polysaccharides from all the strains were found to consist solely of mannose. The purified mannan from each strain was examined for a proton magnetic resonance spectrum, which was recorded on a 60MHz-spectrometer, and for a gel-diffusion precipitation pattern against antisera to the Candida strains. The results of both examinations indicated that the mannans obtained from the Candida strains could be classified into 3 groups on the basis of the difference in chemical structure. The first group comprised C. albicans A-I, C. albicans A-II, C. tropicalis, and C. guilliermondii, the second group C. albicans B, C. stellatoidea, and C. parakrusei, and the third group C. krusei. Therefore, the proton magnetic resonance spectrum of Candida mannan was found to have a close relationship to immunological specificity.
