Abstract
The relationship between the optimal concentration of an adjuvant to be added and the preservationof lyophilized immune serum was examined.
1. The solubility of lyophilizedimmune serum was improved by the previous addition of 4 to 12per cent of glycine or sodium glutamate as adjuvant.
Neither solubility nor potency of purified lyophilized immune horse serum containing 4 per centof glycine underwent any detectable change even after storage at room temperature for three to sevenyears.
2. Ordinary lyophilization resulted in partial insolubility of fluorescein-labeled anti-human IgG rabbit serum and in the appearance of nonspecific fluorescence of the serum. These disadvantageswere eliminated by addition of either of those adjuvants to the material. Even after the labeledserum lyophilized under such conditions were kept at 37 C for a month, no detectable changes wereobserved in the solubility or potency of the serum.
