Nippon Saikingaku Zasshi

[title in Japanese]

[in Japanese]

1974 Volume 29 Issue 4 Pages 651-663
Published: July 25, 1974
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.29.651

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[title in Japanese]

[in Japanese]

1974 Volume 29 Issue 4 Pages 665-669
Published: July 25, 1974
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.29.665

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Studies on the Solubility of Lyophilized Immune Serum

Effect of Adjuvant on Solubility and Preservation

Hideo ADACHI

1974 Volume 29 Issue 4 Pages 671-680
Published: July 25, 1974
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.29.671

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The relationship between the optimal concentration of an adjuvant to be added and the preservationof lyophilized immune serum was examined.
1. The solubility of lyophilizedimmune serum was improved by the previous addition of 4 to 12per cent of glycine or sodium glutamate as adjuvant.
Neither solubility nor potency of purified lyophilized immune horse serum containing 4 per centof glycine underwent any detectable change even after storage at room temperature for three to sevenyears.
2. Ordinary lyophilization resulted in partial insolubility of fluorescein-labeled anti-human IgG rabbit serum and in the appearance of nonspecific fluorescence of the serum. These disadvantageswere eliminated by addition of either of those adjuvants to the material. Even after the labeledserum lyophilized under such conditions were kept at 37 C for a month, no detectable changes wereobserved in the solubility or potency of the serum.

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Studies on Biological Characteristics of Lactobacillus

I. A Modified Method for the Antibiotic Susceptibility Test of Lactobacillus

Kiyoshi TOHYAMA, Tsuneo TERASHIMA

1974 Volume 29 Issue 4 Pages 681-689
Published: July 25, 1974
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.29.681

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A modified single-disc diffusion method was devised for determination of the antibiotic susceptibilityof Lactobacillus by using the soft agar overlayer technique, which had been originally designed forphage titration. In this method, all the test strains of Lactobacillus could grow well on soft agar.
The minimum inhibitory concentrations (MIC), as determined by the modified disc method, ofsix antibiotics (tetracycline, lincomycin, erythromycin, oleandomycin, penicillin G, and chloramphenicol) disagreed with those determined by the agar and tube dilution methods, but were very close to themaximum tolerant concentrations (MTC) for visible growth estimated by the latter methods. Thosedata were obtained successfully by using constant inocula (0.1 ml) of overnight cultures with 130-160Klett units.
A total of 34 strains of Lactobacillus were tested for in vitro sensitivity to 17 antibiotics. As aresult, the antibiotics of the streptomycin group were inactive against all the test strains, except L.acidophilus and L. jugurti. The antibiotics of the macrolide group, mikamycin, penicillin G, andtetracycline were the most active against almost all the strains. The sensitivity to cephalothin variedwith the species and was low in L. casei. The sensitivity of the test strains to 4 antibiotics (cephaloridine, novobiocin, dehydroxymethylfractoridine, and lincomycin) ranged widely from high torelatively low.

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Studies on Biological Characteristics of Lactobacillus

II. Tolerance of the Multiple Antibiotic Resistant Strain, L. casei PSR3002, to Artificial Digestive Fluids

Yohichi KOBAYASHI, Kiyoshi TOHYAMA, Tsuneo TERASHIMA

1974 Volume 29 Issue 4 Pages 691-697
Published: July 25, 1974
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.29.691

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The tolerance of the multiple antibiotic resistant strain, Lactobacillus casei PSR3002, to artificialdigestive fluids and to sodium deoxycholate was compared in vitro with that of its parent strain (L.caseiYIT0091), other lactobacilli, streptococci and bifidobacteria.
The tolerance of L. casei PSR3002 to both artificial intestinal juice and artificial gastric juice wassimilar to that of L. casei YIT0091. Three-hour cultures of the two strains were studied forsusceptibility to artificial gastric juice at various pH values. At pH value above 2.7, there was littledifference between the two strains. At pH 2.5, however, in viable cell count both strains showed amarked decrease.
The higher the concentration of bile, the lower the viable cell count of the two strains. Thenthe elongation of the cells was recognized microscopically.
L. casei PSR3002 showed a longer lag-phase and slower growth than L. casei YIT0091 whenincubated in the artificial intestinal juice after exposure to the artificial gastric juice at pH 2.7 andpH 2.5. The most tolerant to the artificial gastric juice were 4 strains of L. casei and 2 strains of L.acidophilus, which were followed by L. plantarum YIT0062 and Streptococcus faecalis ATCC10541.
The most resistant to sodium deoxycholate solution were S. faecalis ATCC10541, L. casei YIT0091, L. casei PSR3002, L. casei 1-136, L. plantarum YIT0062, and L. acidophilus A-34. Their 50%growth inhibitory concentrations ranged from 330 to 600 mcg/ml. In contrast, Streptococcus thermophilus YIT2001, L. bulgaricus YIT2001, and L. jugurti LJ-4 were less tolerant to a 50% growthinhibitory concentration within a range from 55 to 90 mcg/ml.

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[title in Japanese]

1974 Volume 29 Issue 4 Pages 699-720
Published: July 25, 1974
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.29.699

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