Abstract
The effects of addition of gall powder to the preculture medium on the spore formation and enterotoxin production by C. perfringens in a sporulation medium were studied.
Preculture in cooked meat medium with gall powder enhanced spore formation by 22 of 23 strains tested. With enterotoxigenic strains, NCTC 8798, 8239, 8238 and wild type IW26, the increase in the gall powder concentration from 0 to 5% and from 0 to 3% in cooked meat medium increased the sporulation rate and the number of spores in the sporulation medium. With 20 wild-type strains, comprising one enterotoxigenic and 19 enterotoxin-negative strains, addition of gall powder to cooked meat medium increased the sporulation rate in the sporulation medium in 95% of the strains.
The preculture in cooked meat medium with gall powder also enhanced the enterotoxin production of the enterotoxigenic strains, NCTC 8798, 8239, 8238 and wild type IW26. The increase in the gall powder concentration from 0 to 5% in cooked meat medium enhanced the enterotoxin production in the sporulation medium.
