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Volume 42 (1987)
- Issue 6 Pages 789-
- Issue 5 Pages 707-
- Issue 4 Pages 627-
- Issue 3 Pages 575-
- Issue 2 Pages 471-
- Issue 1 Pages 51-
Volume 42, Issue 3
Displaying 1-5 of 5 articles from this issue
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Hiroshi AKAEDA, Tadataka TANIGUTI1987 Volume 42 Issue 3 Pages 575-581
Published: May 25, 1987
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe effects of addition of gall powder to the preculture medium on the spore formation and enterotoxin production by C. perfringens in a sporulation medium were studied.
Preculture in cooked meat medium with gall powder enhanced spore formation by 22 of 23 strains tested. With enterotoxigenic strains, NCTC 8798, 8239, 8238 and wild type IW26, the increase in the gall powder concentration from 0 to 5% and from 0 to 3% in cooked meat medium increased the sporulation rate and the number of spores in the sporulation medium. With 20 wild-type strains, comprising one enterotoxigenic and 19 enterotoxin-negative strains, addition of gall powder to cooked meat medium increased the sporulation rate in the sporulation medium in 95% of the strains.
The preculture in cooked meat medium with gall powder also enhanced the enterotoxin production of the enterotoxigenic strains, NCTC 8798, 8239, 8238 and wild type IW26. The increase in the gall powder concentration from 0 to 5% in cooked meat medium enhanced the enterotoxin production in the sporulation medium.View full abstractDownload PDF (768K) -
Hajime IIZIMA, Hisao MURATA1987 Volume 42 Issue 3 Pages 583-587
Published: May 25, 1987
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA crude polysaccharide fraction (CPS) prepared from an alkali-extract of Candida albicans (ATCC 56811) cells was analyzed by thinlayer chromatography, gaschromatography and gasmass spectrometry, and the following results were obtained. (1) Thinlayer chromatography: Rf values of CPS proved to be very close to that of digitoxose. (2) Gaschromatography: The digitoxose-like substance isolated from CPS by thinlayer chromatography was analyzed by gaschromatography. The retention time of the substance closely resembled that of digitoxose. The best results were obtained when CPS was hydrolyzed at 100C for 7hr with 2.5N tri-fluoroacetic acid. (3) Gasmass spectrometry: The digitoxose-like substance isolated from CPS by thinlayer chromatography and highpressureliquid chromatography was analyzed by mass spectrometry. Its mass spectra were the same as that of digitoxose. (4) The above results confirmed that CPS contains digitoxose, and strongly suggest that some cardiac glycoside such as digitoxin and digoxin may exist in the alkali-extract of Candida albicans cells.View full abstractDownload PDF (457K) -
Mayumi FURUKAWA, Sumio ARAI1987 Volume 42 Issue 3 Pages 589-595
Published: May 25, 1987
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe inhibitory effects of peroxidase and catalase on the growth of mycoplasmas were investigated. The addition of peroxidase or catalase to liquid medium inhibited the growth of M. pneumoniae, which produces hydrogen peroxide, but not the growth of an H2O2-non producing strain, M. salivarium. Heated peroxidase (at 100C for 15min) and catalase (at 100C for 10min) abolished their inhibitory effects on the growth of mycoplasmas. In the next experiment, M. pneumoniae and M. salivarium were incubated in acetate buffer (pH 5.0) in the presence of hydrogen peroxide and peroxidase at 37C for 60min, and then in fresh PPLO broth at 37C. The CFUs of M. pneumoniae were reduced by 83% in 5 days and those of M. salivarium by 99% in 2 days of cultivation, as compared with control. These results suggest that the peroxidase-H2O2-halide system might be responsible for a part of the defense mechanisms against mycoplasmal infection.View full abstractDownload PDF (602K) -
Takako IKEDA-FUJITA, Shozo KOTANI, Masachika TSUJIMOTO, Tomohiko OGAWA ...1987 Volume 42 Issue 3 Pages 597-602
Published: May 25, 1987
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSAn immunostimulator agent was partially purified from the phenol-water extracts of M. bovis BCG by fractionation with Sepharose 6B and 4B columns. The agents showed multifold bioactivities, many of which are common to endotoxins. They are pyrogenicity, lethality in galactosamine-loaded mice, induction of tumor-necrosis-factor and interferons α/β in primed mice and immunoadjuvancy enhancing antibody production following in vivo administration, activation of the clotting enzyme cascade of the horseshoe crab, complement activation, and stimulatory effects on murine splenocytes and guinea-pig macrophages in in vitro assay. Although the chemical entity responsible for the above bioactivities has not yet been fully understood, chemical analysis revealed that the bioactive agent is polysaccharide (ca 92%), whose main component sugars are mannose and arabinose, connected with fatty acids (ca 10%), amino acids and amino sugars (ca 3.5% together), containing no detectable amounts of such constitutes characteristic of known immunomodulators of bacterial origin as LPS, cord factors, cell wall peptidoglycans, lipoteichoic acids and biologically active nucleic acids.View full abstractDownload PDF (584K) -
1987 Volume 42 Issue 3 Pages 603-618
Published: May 25, 1987
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (2201K)
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