Abstract
Breeding of Japanese wheat varieties with superior dough quality is essential to increase the production of wheat in Japan. The allelic combination of genes coding the glutenin subunits is one of the key factors influencing the properties of wheat flour. Especially, the low-molecular-weight glutenin subunits encoded by Glu-B3 and Glu-A3, and high-molecular-weight glutenin subunit encoded by Glu-A1 are associated with the visco-elasticity of dough and the white salted noodle-making quality. It is suggested that the application of molecular markers for the selection in wheat breeding programs is very useful for the efficient detection of glutenin subunits alleles. In this paper, we present the usefulness of loop-mediated isothermal amplification (LAMP), which is a useful DNA amplification technique for marker-assisted selection. For the typing of glutenin genes, we developed sets of LAMP primers from the sequences of Glu-B3g, Glu-A3d, and Glu-A1b. The glutenin subunit genes were detected under an isothermal condition of 68°C for one hour by LAMP reaction using 0.2 M betaine and wheat DNA that had been simply extracted using the glass fiber filter paper method.
