Mapping of Disease-Related Genes in Japanese Pear using a Molecular Linkage Map with RAPD Markers

抄録

Molecular linkage maps of disease-related genes in the Japanese pear cultivars ‘Kinchaku’ and ‘Kosui’ with RAPD markers were constructed. Using the pseudotestcross method, two separate maps were constructed from the segregation data of 82 F₁ individuals. The linkage map for ‘Kinchaku’ consisted of 120 loci in 18 linkage groups spanning 768cM, while that for ‘Kosui’ contained 78 loci in 22 linkage groups extending over 508cM. The resistance allele of pear scab and the susceptibility allele of black spot were mapped in different linkage groups in ‘Kinchaku’. Compared with the reports on apple genome mapping, we estimated that these maps covered at least about a half of the total pear genome.