抄録
By data mining of EST databases and screening of a genomic library, the cold stimulated gene Lcs19 was isolated from Italian ryegrass (Lolium multiflorum Lam.). The amino acid sequence of the putative LCS19 was 74% identical to wheat (Triticum aestivum) WCS19. Transcripts of Lcs19 were detected after 8 h of cold treatment, and continued to increase up to 48 h after exposure. Lcs19 expression was induced only by cold temperature, and not by high salinity or exogenous abscisic acid. No Lcs19 expression was observed in seedlings that underwent the cold treatment in the dark. Comparison of the promoter of Lcs19 with that of homologous genes of wheat and barley (Hordeum vulgare) showed that regions that include the DRE/CRT cis-acting element sequence were conserved. These results suggest that Lcs19 expression is regulated by signal pathway mediated by the DRE/CRT element and other signal pathways reflecting photosynthetic conditions such as a redox signal.
