Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)

Cina Ann Vipin; David J. Luckett; John D.I. Harper; Gavin J. Ash; Andrzej Kilian; Simon R. Ellwood; Huyen T.T. Phan; Harsh Raman

doi:10.1270/jsbbs.63.292

Research Papers

Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)

Cina Ann Vipin, David J. Luckett, John D.I. Harper, Gavin J. Ash, Andrzej Kilian, Simon R. Ellwood, Huyen T.T. Phan, Harsh Raman

Author information

Cina Ann Vipin
Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga Agricultural Institute
David J. Luckett
Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga Agricultural Institute
John D.I. Harper
Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga Agricultural Institute
School of Agricultural and Wine Sciences, Charles Sturt University
Gavin J. Ash
Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga Agricultural Institute
School of Agricultural and Wine Sciences, Charles Sturt University
Andrzej Kilian
Diversity Arrays Technology P/L
Simon R. Ellwood
Department of Environment and Agriculture, Curtin University
Huyen T.T. Phan
Department of Environment and Agriculture, Curtin University
Harsh Raman
Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga Agricultural Institute

Keywords: Lupinus albus, genetic linkage mapping, molecular markers, Diversity Array Technology

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Supplementary material

2013 Volume 63 Issue 3 Pages 292-300

DOI https://doi.org/10.1270/jsbbs.63.292

Details

Abstract

We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F₈ recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genic markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good genome coverage and were associated with previously identified genic and AFLP markers linked with quantitative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies.

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