Breeding Science
Research Papers
Construction of a linkage map based on retrotransposon insertion polymorphisms in sweetpotato via high-throughput sequencing
Yuki MondenTakuya HaraYoshihiro OkadaOsamu JahanaAkira KobayashiHiroaki TabuchiShoko OnagaMakoto Tahara
Author information
JOURNALS FREE ACCESS FULL-TEXT HTML

Volume 65 (2015) Issue 2 Pages 145-153

Details
Full Text-HTML Download PDF (617K) Contact us
Abstract

Sweetpotato (Ipomoea batatas L.) is an outcrossing hexaploid species with a large number of chromosomes (2n = 6x = 90). Although sweetpotato is one of the world’s most important crops, genetic analysis of the species has been hindered by its genetic complexity combined with the lack of a whole genome sequence. In the present study, we constructed a genetic linkage map based on retrotransposon insertion polymorphisms using a mapping population derived from a cross between ‘Purple Sweet Lord’ (PSL) and ‘90IDN-47’ cultivars. High-throughput sequencing and subsequent data analyses identified many Rtsp-1 retrotransposon insertion sites, and their allele dosages (simplex, duplex, triplex, or double-simplex) were determined based on segregation ratios in the mapping population. Using a pseudo-testcross strategy, 43 and 47 linkage groups were generated for PSL and 90IDN-47, respectively. Interestingly, most of these insertions (~90%) were present in a simplex manner, indicating their utility for linkage map construction in polyploid species. Additionally, our approach led to savings of time and labor for genotyping. Although the number of markers herein was insufficient for map-based cloning, our trial analysis exhibited the utility of retrotransposon-based markers for linkage map construction in sweetpotato.

Information related to the author
© 2015 by JAPANESE SOCIETY OF BREEDING
Previous article Next article

Recently visited articles
feedback
Top