抄録
A method for high-performance liquid chromatography of carbohydrates was developed by using Hitachi 2633 and 2634 strong anion exchange resins of borate form, and Fluoresugar (acyanoacetamide) as the fluorogenic detecting reagent. Ten carbohydrates were completely separated in 100min by stepwise elution with two borate buffers, and the lower limit of detection was 10 picomole for each carbohydrate.
For routine analysis of the monosaccharide compositions of carbohydrate materials in sera and urines, a single-buffer system was used, which allowed rapid determination of xylose, fucose, galactose, glucose, and mannose. The fucose/galactose molar ratios for the non-dialyzable urinary carbohydrate materials of several cancerous patients were found to be lower than those of normal subjects.
