A-4. 赤血球膜酵素と病態生化学

抄録

Cytoplasmic enzymes from red blood cells are frequently assayed in clinical studies. but the use of membranes is rare. The latter are composed mainly of insoluble proteins, are rich in lipid, and are easily broken. The three ATPase,(Mg),(Na+K+Mg),(Ca+Mg) ATPase are incorporated into the membrane where they related to other components, and constitute a part of the membrane. Usually the active center of these ATPase are masked and show little activity. Depending on the treatment used, the total detectable ATPase activity is variable. Studies of the (Na+K+Mg) ATPase of the red blood cells in muscular dystrophy for example, have produced variable results.
In order to detect the high activities of the ATPase, freezing-thawing treatment was here used. With acetylcholine esterase which is said to be on the outer side of red blood cell membrane and aldolase which is said to be on the inner side, no increase of the activities was found after the treatments. However, the effect of freezing-thawing on the distribution of two membranous enzymes varied. The differences between patients with Duchenne type muscular dystrophy and normals on these activities and distribution of two enzymes could not be caught.
The ATP hydrolysed by the hemolysate appeared to be due to the membranous ATPase because hemolysate and washed membranes showed a similar cation activation profile of ATPase activities. The activities of the hemolysate ATPase from patients with Duchenne type and their mothers were lower than that from their fathers, although the values of their mothers were variable. After the cytoplasma was added to the membrane, the activities of the membranous ATPase from patients showed a smaller increase than that from their mothers.
As a result of it, the red blood cell from patient seems to wear a disorder-a membrane disorder.