Three aspects were under consideration through daily experiences.
Regarding to unstable HbA1 in microcolumn chromatography, further improvements in accuracy and specificity should be rather required, otherwise at least in present method, there were no significances in removing unstable HbA1 by pretreatment of erythrocytes.
In the cases of HbA1 elevation aside from diabetes mellitus, determinations of HbA1 subcomponents were desirable and subsidiary index of HbA1a+b/HbA1c ratio was available.
From long-term follow-up study, it was demonstrated that HbA1c has proved to be more useful than FBS in assessing the degree of patient control, especially under insulin therapy.
