B-9虚血性脳血管障害における血漿トロンボキサンB2の測定
1982 年 21 巻 p. 63-67
詳細
1982 年 21 巻 p. 63-67
Plasma thromboxane B2 (TXB2) in ischemic cerebrovascular diseases and other diseases were measured and its relation to the period after onset (or last attack) and to aspirin (ASP) treatment was studied. Platelet aggregation was examined simultaneously in some cases.
Object: For TXB2, transient ischemic attack (TIA) 8 cases (11 measured), cerebral infarction 18 cases (19 measured), cerebral bleeding 2 cases, epilepsy 2 cases and normal healthy control 24 cases were measured. For platelet aggregation, 4 cases of TIA, 8 cases of cerebral infarction and other 3 cases were examined.
Assay methods: TXB2 was extracted from heparinized plasma by ethyl acetate, purified by thin layer chromatography and assayed by radioimmunoassay. Platelet aggregation was examined from sodium citrate-mixed blood by absorption method for ADP (2×10-5M) and collagen (3μg/ ml)
Result: Plasma TXB2 (mean±SD) was as follows, control: 155±35pg/ml; TIA: ASP nontreated: 260±215 (6 measurement), ASP treated: 52±41 (4); cerebral infarction: within one month after onset: ASP non-treated: 1750±1310 (4), ASP treated: 42 (2), over one month after onset: ASP non-treated: 59±35 (7), ASP treated: 64±16 (3); cerebral bleeding: 6 days after onset: 1120, 9 months after onset: 165, epilepsy: one day after attack: 626, one month after attack: 298.
In eschemic cerebrovascular diseases (TIA and cerebral infarction) logarithmically converted values of both TXB2 and the period (days) after onset were correlated signifcantly (correlation coefficient -0.77, N=15, p<0.01). Seven paired TXB2 values of ASP non-treated and treated cases, measured at the same time after onset, were selected. Their means and SD were 236±205 in ASP non-treated and 51±23 in ASP treated cases. It's difference was not significant. 2 cases over 90% absorption in platelet aggregation for both ADP and collagen (TIA and epilepsy) gave high TXB2 values (3320 and 298 respectively) and other 13 cases under 90% had normal values.
Conclusion: Plasma TXB2 increased in ASP non-treated cases of TIA and those of cerebral infarction within one month after onset. Each case after cerebral bleeding and convulsion gave also high plasma TXB2 values. Increased platelet aggregation was found in 2 cases showing high plasma TXB2 values.
