臨床化学
Online ISSN : 2187-4077
Print ISSN : 0370-5633
ISSN-L : 0370-5633
血清, 組織のドーパミンβ-水酸化酵素の活性測定法
加藤 武葛谷 博磁永津 俊治岡田 保
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1972 年 1 巻 2 号 p. 198-204

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Details of an enzymatic radioassay procedure ofdopamine-β-hydroxylase in serum and tissues have been described. The method is based on the formation of octopamine from tyramine by dopamine-β-hydroxylase and a subsequent conversion of octopamine to N-14C-methyl-octopamine by the second incubation with S-adenosyl-L-methionine-(methyl-14C) and phenylethanolamine-N-methyltransferase. The normal value of human serum and examples of the activities of human sera in various diseases, as well as the activities in rat sera have been shown.
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