肝硬変患者血清にみられる小腸性アルカリホスファターゼ
アイソザイムの分子多様性 (I)
1981 年 10 巻 1 号 p. 40-46
詳細
1981 年 10 巻 1 号 p. 40-46
This papar presents the existance of higher molecular form of intestinal aikaline phosphatase isozyme (ALP, EC 3.1.3.1) in serum of patients with liver cirrhosis.
The ALP in the patient's serum was separated into two different molecuiar weight forms, 7S and 19S-7Sprotein fractions, by Sephadex G-200gel filtration.The apparent molecuiar weight of the 7S and 19S-7S ALP fractions was estimated to be about 200,000 and 350,000, respectively, from the elution voiume of the chromatography.The size of the 19S-7S ALP fraction was much smaller than that of so called high-molecular-weight liver isozyme in serum of patients with choiestatis.
The 19S-7S ALP fraction was remarkably inhibited by L-phenylalanine, and the properties of amino acids inhibition were closely resemble to intestinal isozyme.The treatment of 19S-7S ALP fraction with Triton X-100 caused the partial decrease of its apparent molecular weight.
The results suggest that the 19S-7S ALP fraction is a complex of intestinal isozyme and lipid.
