Abstract
Comparative study of Amberlite XAD7 and Amberlite XAD2 on the extraction of individual serum bile acids was observed.lndividual serum bile acids was determined by a highly sensitive determination of bile acids using high-performance liquid chromatography combined with enzymatic fluorometric measurement, which was previously reported by us (S.Baba et al.Kobe J.Med.Sci., 26: 89, 1980).
The extraction rate of each bile acid using Amberlite XAD7 was higher than that of Amberlite XAD2.Therefore, the minimum detectable limits of each bile acid, especially taurine-conjugated bile acids by using Amberlite XAD7, was observed more sensitive and accurate than that of Amberlite XAD2.