臨床化学
Online ISSN : 2187-4077
Print ISSN : 0370-5633
ISSN-L : 0370-5633
速度法による基質濃度の酵素的測定法成立条件の基礎的検討
等々力 徹松本 宏治郎加野 象次郎
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1981 年 10 巻 4 号 p. 325-331

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Theoretical considerations on enzymatic rate analysis for substrate determination were examined.In the case of enzymatic rate analysis, a dynamic range is decided by Km value of the using enzyme and allowable relative error. When allowable relative error is setted to one per-cent, a dynamic range is less than 1/100Km of the enzyme.Initial velocities are also determined by differential coefficient at zero time of exponential regression formula taken from the reaction time course and the results are in good agreement with the velocities derived from theoretical formula.These theoretical considerations were experimentally established in the system of ammonium ion assay with glutamate dehydrogenase.

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