抄録
An enzymic method for the determination of inorganic phosphate in urine was established, based on the formation of hydrogen peroxide with purine nucleoside phospho rylase (PNP) and xanthine oxidase (XO).
Inosine+H3PO4 PNP→hypoxanthine+ribose-1-phosphate Hypoxanthine+2H2O+2O2XO→uric acid+2H2O2 H2O2+red. chromogen peroxidase→oxid. chromophore+ H2O
The method was evaluated by the investigation of its precision, accuracy, sensitivity and specificity. It was concluded that this method was useful in clinical tests for the determi nation of inorganic phosphate in urine.
