Fosfomycin (FOM) is an antibiotic drug of low molecular weight, exhibiting antibacterial activity against gram-negative and positive bacteria.FOM has no distinct absorption in the UV spectrum. This paper describes a highly sensitive and speedy method for the determination of FOM in human serum. This measurement was achieved by highperformance liquid chromatography with refractive index detection. Only a 50μl of senum was enough for this method.lt took 8 minutes to analyze FOM in the sample, using a silica-NH column and 0.08M NaC1-0.1M phosphate buffer (pH 2.0) as mobile phase. The detection limit of this method was 100ng FOM. The results demonstrated that the proposed method is suitable for the detrmination of FOM in human serum.
