Abstract
Highly sensitive enzyme immunoassay using chemiluminescence reaction has been developed.Glucose oxidase was used as the labding enzyme and conjugated with anti-α-fetoprotein lgG.
Free and bound fractions after immune reaction, were separlted by insoiubilized antia-fetoprotein lgG.The enzyme activity was measured by chemiluminescence reaction.Hydro gen peroxide, generlted from glucose by glucose oxidlse reacts with isoluminol-microperoxidase system to produce chemiluminescence The luminescence intensity was linearly propotional to glucose oxidase activit.
The coupled reaction was used as a sensitive precise micro method for the determination of α-fetqprotein.The range of detection was 3-10ng/ml α-fetoprotein.
