Abstract
A highly sensitive fluorescence assay for total bile acid in serum using a flow injection system is described.
The applrltus consists of six-port valve, pretreatment column (2×20mm) packed serumout-25 (Sekisui chemical co.), immobilized enzyme column (3α-hydroxysteroid dehydrogenase, 3α-HSD, 2×30mm), injector, peristaltic pump and fluorescence detector.
This system enables to detect total bile acid from serum injection directly without sample pretreltment.The new device is especially useful for clinical studies where total bile acid has to be determined from serum.Moreover, the method was more sensitive and simple than conventional method.(cv=1.99%, n=10).
