抄録
A highly specific assay for the plasma kallikrein complex withα-macroglobulin has been developed.Plasma kallikrein-α-macroglobulin complex was quantitatively trapped by use of the polystyrene bead coated with the specific anti-α-macroglobulin antibody, and then the plasma kallikrein activity on the bead was determirned with Pro-Phe-Arg-4-methylcoumaryl-7-amide as a substrate.
The proposed method had a good precision. The detection range of plasma kallikrein-α2-macroglobulin complex was from 3 to 100 U/l. By the proposed method, the activity of plasma kallikrein in the complex in normal serum was found to be 44.8±3.7 U/l and the serum activity was significantly decreased in the patients with eiver cirrhosis.
