ブタ卵巣を利用した簡単なhCGのラジオレセプターアッセイの試み

抄録

A radioreceptor assay (RRA) system for a simple and rapid determination of human serum chorionic gonadotropin (hCG) 1evel was developed by using a receptor preparation from porcine ovaries.The system mainly consists of a hCG-receptor-activity containing fraction, which was obtained from porcine ovary homogenate through the differential centrifugation, and a [¹²⁵1] -labeled hCG.A serum hCG level more than 0.025 lU/ml was able to be determined by this method within a 30 min-incubation and the coefficient of variation was at 8.6%.Serum hCG levels from individuals of different gestational periods were estimated by the method, showing a peak around 10 gestational weeks, gradually decreased and again representing a second peak around 30-35 weeks.The hCG levels thus obtained were compared with those obtained by the radioimmunoassay (RIA) with a NlH kit.Both values were significantly correlated (r=0.501, P<0.001) with each others.