Abstract
A simple and sensitive method for direct determination of whole-blood iron without deproteinization is described. Whole-blood iron was split and oxidized by the treatment of diluted blood with periodic acid solution. Ascorbic acid solution was then added to this solution to reduce the oxidized iron (Fe3+) to Fe2+. 2-Nitoroso-5-(N-propyl-N-sulfopropylamino) phenol solution added to the reacted mixture formed a complex with Fe2+ which exhibited a peak absorption at 745nm by addition of ammonium acetate solution. The method was suited to the determination of whole-blood iron at concentrations of 1-100mg/dl. The C. V. in within-run precision was 1.83% with the recovery of 101.7%. A close correlation was found between this method and other employed methods.
