Abstract
A new sensitive and simple method for color-developing determination of plasma total bilirubin is described. This method is based on oxidative reaction involving formaldehyde. In the presence of hydrogen peroxide, peroxidase in horseradish shows a peroxidase activity and catalyzes the formation of a deep greenish blue dye with an absorption peak at 615 nm, caused by the oxidative reaction with bilirubin and formaldehyde. The proposed method using 5% solution of Triton X-100 in 0.1 M phosphate buffer (pH 7.0) eliminated the interferences derived from blood components. The method was suited to the measurement of bilirubin at concentrations of 0.5-40.0 mg/dl. The C. V. in within-run precision was 1.41% with the recovery of 105.3%. A close correlation was found between this and other employed methods.
