抄録
A method for the determination of bile acids in normal serum specimen was investigated using a high-performance liquid chromatograph equipped with an automated pretreatment system (Serumout-25, Sekisui Chemical Co.). The pretreatment system could serve for purification and concentration of bile acids in serum within a few minutes. So, it would be possible to determine their contents in normal serum by the system. The presence of 6-12 kinds of bile acids (ursodeoxycholic acid, cholic acid, glycoursodeoxycholic acid, glycocholic acid chenodeoxycholic acid, deoxycholic acid, glycochenodeoxycholic acid, glycodeoxycholic acid, taurochenodeoxycholic acid, taurodeoxycholic acid, lithocholic acid, glycolithocholic acid) was demonstrated in serum specimens from healthy individuals. In these cases, chenodeoxycholic acid and glycochenodeoxycholic acid were always detected, but tauroursodeoxycholic acid and taurolitholic acid were not. This method has proved to be free from the interference of drugs and other constituents in serum, and therefore, is suitable for the reliable determination of bile acids in biological samples.
