高速液体クロマトグラフィーによる汗のグルコース動態の研究

抄録

Comparison of glucose contents in sweat with blood glucose in 5 healthy subjects is described. After sampling of blood and sweat in the fasting condition, 50g Oral Glucose Tolerance Test (OGTT) was conducted. Blood or sweat was sampled alternately at intervals of 15 minutes for two hours during OGTT. Glucose in blood, sampled from venous of the subjects, was measured using a glucose oxidase enzyme electrode. Glucose in sweat, sampled from the head of them in a steam bath, was analyzed with High Performance Liquid Chromatograph (HPLC).
In the fasting condition glucose contents in sweat and blood were 0.18-0.60 mg/dl and 75-90 mg/dl, respectively. These sweat values were by one to two orders of magnitude lower than those reported previously. This discrepancy may be ascribed to the differences between clasical and modern techniques. The values reported previously may include some erroneous factors such as coexisting reducing substances. HPLC enables us to measure minute changes of actual glucose contents in sweat. No relation was observed between blood glucose and sweat glucose in the subjects. The results of OGTT were classified into two types. The glucose values in blood and sweat increased remarkably to 155-188% and 128-136% at 60 and 45 minutes after oral administration of glucose, respectively, in three of the subjects. On the otherhand, blood glucose hardly increased, but sweat glucose slightly decreased after the administration in two of them. Glucose content in sweat comparatively reflects blood glucose, but may relate to not only blood glucose but also individual difference and situation of perspiration such as sweating rate. Blood glucose might be estimated noninvasively using a correlation between blood and perspiration, if a statistically significant relationship between the two could be established.